Coding

Part:BBa_K929302:Design

Designed by: Potsdam Bioware 2012 iGEM   Group: iGEM12_Potsdam_Bioware   (2012-09-24)


AID in Potsdam Standard


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 84
    Illegal SapI site found at 185


Design Notes

AID was amplified with primers which contains thiophosphates at 5' end and insert in Potsdam Standard Backbone using PLIcing method.


Source

BBa_K103001

References